Vertical distribution of major sulfate-reducing bacteria in a shallow eutrophic meromictic lake.
نویسندگان
چکیده
The vertical distribution of sulfate-reducing bacteria was investigated in a shallow, eutrophic, meromictic lake, Lake Harutori, located in a residential area of Kushiro, Japan. A steep chemocline, characterized by gradients of oxygen, sulfide and salinity, was found at a depth of 3.5-4.0 m. The sulfide concentration at the bottom of the lake was high (up to a concentration of 10.7 mM). Clone libraries were constructed using the aprA gene, which encodes adenosine-5'-phosphosulfate reductase subunit A, in order to monitor sulfate-reducing bacteria. In the aprA clone libraries, the most abundant sequences were those from the Desulfosarcina-Desulfococcus (DSS) group. A primer set for a DSS group-specific 16S rRNA gene was used to construct another clone library, analysis of which revealed that the uncultured group of sulfate-reducing bacteria, SEEP SRB-1, accounted for nearly half of the obtained sequences. Quantification of the major bacterial groups by catalyzed reporter deposition-fluorescence in situ hybridization demonstrated that the DSS group accounted for 3.2-4.8% of the total bacterial community below the chemocline. The results suggested that the DSS group was one of the major groups of sulfate-reducing bacteria and that these presumably metabolically versatile bacteria might play an important role in sulfur cycling in Lake Harutori.
منابع مشابه
Vertical distribution of major sulfate-reducing bacteria in a eutrophic shallow meromictic lake
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Title Vertical distribution of major sulfate-reducing bacteria in a shallow eutrophic meromictic lake Author(s) Kubo, Kyoko; Kojima, Hisaya; Fukui, Manabu Citation Systematic and Applied Microbiology, 37(7): 510-519 Issue Date 2014-10 DOI Doc URL http://hdl.handle.net/2115/57661 Right Type article (author version) Additional Information There are other files related to this item in HUSCAP. Chec...
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ورودعنوان ژورنال:
- Systematic and applied microbiology
دوره 37 7 شماره
صفحات -
تاریخ انتشار 2014